GM 501 PVP

GM501 PVP is a ready-to-use medium to reduce the motility of sperm, making it easier to catch them with an ICSI pipette.

Composition:

• GM501 PVP is 10 % dialysed PhEur grade polyvinylpyrrolidone solved in an aqueous solution containing physiologic salts, HEPES, lactate, pyruvate, glucose and human albumin solution.

Instructions for use:

• Evaporation of the PVP solution on the slide or dish and the presence of human albumin may occasionally cause a small amount of precipitation in the medium.

Standard procedure:

1. Warm the PVP solution to 37°C.
2. Place a small drop of PVP solution (5 μl – 10 μl) in a dish and cover with GM501 MineralOil.
3. Add a small volume (1 μl – 2 μl) of washed sperm into the centre of the PVP droplet.
4. Wait for a few minutes to allow sperm to migrate to the periphery of the droplet.
5. Select and recover the spermatozoa for injection.