Lykos Laser with DTS

The Newest in Laser Technology for Clinical Applications

• 300 mW laser power and one microsecond pulse length
• Laser and RED-i® target locator built into 40x objective
• Multi-pulse mode for fast and easy trophectoderm biopsy
• High image quality
• Improved working distance - use with or without glass heater
• No laser alignment needed
• Similar in size to standard objectives
• Compatible with all major microscope models

Laser and RED-i® Target Locator built into 40x Objective

Laser

The LYKOS® features a 1460 nm, 300mW Class 1 laser built-into a custom designed objective 40x objective.

Objective Specifications:

• 40x, 0.60 N.A., I.R.
• High transmission in the UV [>350 nm], visible and near-infrared, long working distance
• Infinity corrected for 1 mm thick polystyrene Petri Dish, on 500 μm glass heating plate. A nose cap corrector is available if use without the glass heating plate is required.
• Working Distance: 1.7 mm to glass heater plate
• Parfocality: < 40 µm
• Compatibility: 3 RMS thread adapters permit installation on all major microscope models

RED-i®

The RED-i speeds workflow by allowing you to position the cell under the laser beam without looking at the monitor.

• Built into the LYKOS laser objective
• Red LED indicator spot visible through microscope eyepieces
• Adjustable brightness level
• Always remains in focus
• Laser beam is NOT transmitted through eyepieces - SAFE for your eyes
• Simple alignment process

Introducing the Multi-Pulse Feature for the LYKOS Laser System

The Multi-Pulse software gives our lasers an automated, precise tool that enables rapid trophectoderm biopsy.

The Multi-Pulse feature provides rapid, repeated firing of the laser to facilitate removal of cells from an embryo during the trophectoderm biopsy process. Trophectoderm biopsy is considered one of the best methods used to remove cells from the embryos of patients undergoing pre-implantation genetic diagnosis (PGD) to screen for genetic disease or aneuploidy.

Prior to release of the Multi-pulse software, individual laser shots were required to weaken or break the junctions between the trophectoderm cells so that they can be aspirated into the biopsy micropipette. The significant advantage of Multi-Pulse is that one press of the remote footswitch initiates multiple laser pulses in rapid succession for fast and easy cell separation, thereby limiting the amount of time the embryo spends outside the incubator.

Professor Barry Behr, Co-Director, REI/IVF Program, Department of Obstetrics and Gynecology at the Stanford University School of Medicine, a long-time user of Hamilton Thorne lasers and a pioneer in trophectoderm biopsy, commented, “The Multi-Pulse capability, which provides the user with the ability to automatically fire the laser multiple times at relatively low power, will contribute significantly to the efficiency and safety of trophectoderm biopsy.”

With precise targeting features, automated calibration and built-in quality control reporting, LYKOS with DTS provides computer-controlled accuracy and enhanced ease-of-use. With the laser and RED-i built into the LYKOS objective, the LYKOS with DTS offers portability, easy installation, and out-of-the-box compatibility with all current models of inverted microscopes.

Computer-controlled Accuracy and Ease of Use through:

• Precise Targeting Features
• Automated Calibration
• Built-in Quality Control Reporting

The Dynamic Targeting System (DTS) option for the LYKOS represents the next generation of clinical lasers for Assisted Reproductive Technology. With precise targeting features, automated calibration and built-in quality control reporting, LYKOS with DTS provides computer-controlled accuracy and enhanced ease-of-use. With the laser and RED-i built into the LYKOS objective, the LYKOS with DTS offers portability, easy installation, and out-of-the-box compatibility with all current models of inverted microscopes.

Advanced Targeting Features

The DTS feature is available in various markets worldwide. Depending on the market and application, the DTS feature may be enabled in both Clinical* and Validation** Modes, or Validation Mode only. When DTS is enabled, you may choose one of several options to create the laser path:

• Single Shot: Click any single point on the screen and a single laser pulse will be applied to that exact point.
• Line / Curve: Draw a straight line of any length and then select and drag to create a curve.
• Freehand: Any freeform path may be drawn by clicking and dragging the mouse pointer.
• Multipoint: Click up to 10 non-connected locations to apply laser. Each point may have a different laser setting.
• Rectangle: Click and drag to draw a rectangle of any proportion.

Once the path is drawn, it may be selected, resized, and repositioned as needed. Depending on the selected mode, the laser pulse, power and/or spacing may be adjusted with the changes immediately reflected in the laser path on the screen.

Automated Calibration

The automated initialization process calibrates the system and can be run any time from the control panel. This initialization process maps the position of the RED-i target in relation to the entire field of view to optimize accuracy.

Built-in Quality Control Reporting

Quality control is a vital part of laboratory procedure. The DTS positioning can be easily verified prior to use and the results of the verification saved. With the built-in quality control reporting, you can view the results of the daily verification in both numerical and graphical form. In addition, a Trend Chart lets you see the results over a selected period of time.